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Product Info Summary
| SKU: | A12628 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-AP-3 complex subunit mu-1 AP3M1 Antibody
SKU/Catalog Number
A12628
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-AP-3 complex subunit mu-1 AP3M1 Antibody (Catalog # A12628). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
AP3M1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-AP-3 complex subunit mu-1 AP3M1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12628)
Host
Rabbit
Contents
AP3M1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
AP3M1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human AP3M1. The immunogen is located within amino acids 340 - 390 of AP3M1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
AP3M1 antibody may cross-react with AP3M2.
Reactive Species
A12628 is reactive to AP3M1 in Human, Mouse, Rat
Applications
A12628 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
46.939kDa
Background of AP3M1
The AP3 complex is a heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. AP3M1 is highly homologous to AP3M2 but is the preferred binding partner to the YQRL motif from the cytosolic domain of TGN38. AP3M1 has also been shown to interact with the HIV-1 virulence protein Nef.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
AP3M1 antibody can be used for detection of AP3M1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of AP3M1 in human brain tissue lysate with AP3M1 antibody at (A) 1 and (B) 2 μg/mL .
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Immunohistochemistry of AP3M1 in mouse brain tissue with AP3M1 antibody at 5 μg/mL.
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Immunofluorescence of AP3M1 in mouse brain tissue with AP3M1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For AP3M1 (Source: Uniprot.org, NCBI)
Gene Name
AP3M1
Full Name
AP-3 complex subunit mu-1
Weight
46.939kDa
Superfamily
adaptor complexes medium subunit family
Alternative Names
adaptor-related protein complex 3, mu 1 subunit; AP-3 adapter complex mu3A subunit; MGC22164; mu-3A subunit; mu3A-adaptin AP3M1 adaptor related protein complex 3 subunit mu 1 AP-3 complex subunit mu-1|AP-3 adapter complex mu3A subunit|adapter-related protein complex 3 mu-1 subunit|adaptor related protein complex 3 mu 1 subunit|clathrin adaptor complex AP3, mu-3A subunit|mu-adaptin 3A|mu3A-adaptin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AP3M1, check out the AP3M1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AP3M1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AP-3 complex subunit mu-1 AP3M1 Antibody (A12628)
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